Mireille Montcouquiol

Publications

• The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling
  Ana Dorrego-Rivas, Jerome Ezan, Maïté M. Moreau, Sonia Poirault-Chassac, Nathalie Aubailly, Julie De Neve, Camille Blanchard, Francis Castets, Amélie Fréal, Arne Battefeld, Nathalie Sans, Mireille Montcouquiol.
  Sci. Adv.. 2022-09-09. 8(36)
  10.1126/sciadv.abo6333
• Neuron-Specific Deletion of Scrib in Mice Leads to Neuroanatomical and Locomotor Deficits
  Jerome Ezan, Maité M. Moreau, Tamrat M. Mamo, Miki Shimbo, Maureen Decroo, Nathalie Sans, Mireille Montcouquiol.
  Front. Genet.. 2022-05-25. 13
  10.3389/fgene.2022.872700
• The cell polarity protein Vangl2 in the muscle shapes the neuromuscular synapse by binding to and regulating the tyrosine kinase MuSK
  Myriam Boëx, Steve Cottin, Marius Halliez, Stéphanie Bauché, Céline Buon, Nathalie Sans, Mireille Montcouquiol, Jordi Molgó, Muriel Amar, Arnaud Ferry, Mégane Lemaitre, Andrée Rouche, Dominique Langui, Asha Baskaran, Bertrand Fontaine, Julien Messéant, Laure Strochlic.
  Sci. Signal.. 2022-05-17. 15(734)
  10.1126/scisignal.abg4982
• Scribble Controls Social Motivation Behavior through the Regulation of the ERK/Mnk1 Pathway
  Maïté M. Moreau, Susanna Pietropaolo, Jérôme Ezan, Benjamin J. A. Robert, Sylvain Miraux, Marlène Maître, Yoon Cho, Wim E. Crusio, Mireille Montcouquiol, Nathalie Sans.
  Cells. 2022-05-10. 11(10) : 1601.
  10.3390/cells11101601
• Vangl2, a Core Component of the WNT/PCP Pathway, Regulates Adult Hippocampal Neurogenesis and Age-Related Decline in Cognitive Flexibility
  Muriel Koehl, Elodie Ladevèze, Mireille Montcouquiol, Djoher Nora Abrous.
  Front Aging Neurosci.. 2022-03-04. 9(14) : 844255.
  10.3389/fnagi.2022.844255
• Early loss of Scribble affects cortical development, interhemispheric connectivity and psychomotor activity.
  Jerome Ezan, Maité M. Moreau, Tamrat M. Mamo, Miki Shimbo, Maureen Decroo, Melanie Richter, Ronan Peyroutou, Rivka Rachel, Fadel Tissir, Froylan Calderon de Anda, Nathalie Sans, Mireille Montcouquiol.
  Sci Rep. 2021-04-27. 11(1)
  10.1038/s41598-021-88147-1
• Vangl2, a core component of the WNT/PCP pathway, regulates adult hippocampal neurogenesis and age-related decline in cognitive flexibility
  M Koehl, E Ladevèze, M Montcouquiol, DN Abrous.
  . 2021-01-06.
  10.1101/2021.01.05.425435
• Muscle Van Gogh-like 2 shapes the neuromuscular synapse by regulating MuSK signaling activity
  Myriam Boëx, Julien Messéant, Steve Cottin, Marius Halliez, Stéphanie Bauché, Céline Buon, Nathalie Sans, Mireille Montcouquiol, Jordi Molgó, Muriel Amar, Arnaud Ferry, Mégane Lemaitre, Andrée Rouche, Dominique Langui, Asha Baskaran, Bertrand Fontaine, Laure Strochlic.
  Preprint bioRxiv. 2020-11-27.
  10.1101/2020.11.26.384925
• Scribble controls social behaviors through the regulation of the ERK/Mnk1 pathway
  Maïté M. Moreau, Susanna Pietropaolo, Jérôme Ezan, Benjamin J.A. Robert, Sylvain Miraux, Marlène Maître, Yoon Cho, Wim E. Crusio, Mireille Montcouquiol, Nathalie Sans.
  . 2020-09-11.
  10.1101/2020.09.10.289397
• Vangl2 in the Dentate Network Modulates Pattern Separation and Pattern Completion
  Benjamin J.A. Robert, Maïté M. Moreau, Steve Dos Santos Carvalho, Gael Barthet, Claudia Racca, Mehdi Bhouri, Anne Quiedeville, Maurice Garret, Bénédicte Atchama, Alice Shaam Al Abed, Christelle Guette, Deborah J. Henderson, Aline Desmedt, Christophe Mulle, Aline Marighetto, Mireille Montcouquiol, Nathalie Sans.
  Cell Reports. 2020-06-01. 31(10) : 107743.
  10.1016/j.celrep.2020.107743
• Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth
  Steve Dos-Santos Carvalho, Maite M Moreau, Yeri Esther Hien, Michael Garcia, Nathalie Aubailly, Deborah J Henderson, Vincent Studer, Nathalie Sans, Olivier Thoumine, Mireille Montcouquiol.
  eLife. 2020-01-07. 9
  10.7554/eLife.51822
• Selective neurodevelopmental and behavioral deficits in Scrib conditional knock-out mice recapitulate some phenotypes associated with the Verheij/8q23.4 deletion syndrome
  Jerome Ezan, Maité M. Moreau, Tamrat M. Mamo, Miki Shimbo, Maureen Decroo, Melanie Richter, Ronan Peyroutou, Rivka Rachel, Fadel Tissir, Froylan Calderon de Anda, Nathalie Sans, Mireille Montcouquiol.
  . 2019-09-23.
  10.1101/780130
• Development and Patterning of the Cochlea: From Convergent Extension to Planar Polarity.
  Mireille Montcouquiol, Matthew W. Kelley.
  Cold Spring Harb Perspect Med. 2019-01-07. : a033266.
  10.1101/cshperspect.a033266
• Gαi Proteins are Indispensable for Hearing.
  Sandra Beer-Hammer, Sze Chim Lee, Stephanie A. Mauriac, Veronika Leiss, Isabel A. M. Groh, Ana Novakovic, Roland P. Piekorz, Kirsten Bucher, Chengfang Chen, Kun Ni, Wibke Singer, Csaba Harasztosi, Thomas Schimmang, Ulrike Zimmermann, Klaus Pfeffer, Lutz Birnbaumer, Andrew Forge, Mireille Montcouquiol, Marlies Knipper, Bernd Nürnberg, Lukas Rüttiger.
  Cell Physiol Biochem. 2018-01-01. 47(4) : 1509-1532.
  10.1159/000490867
• The embryonic development of hindbrain respiratory networks is unaffected by mutation of the planar polarity protein Scribble.
  Marc Chevalier, Laura Cardoit, Maïté Moreau, Nathalie Sans, Mireille Montcouquiol, John Simmers, Muriel Thoby-Brisson.
  Neuroscience. 2017-08-01. 357 : 160-171.
  10.1016/j.neuroscience.2017.05.046
• Defective Gpsm2/Gαi3 signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome.
  Stephanie A. Mauriac, Yeri E. Hien, Jonathan E. Bird, Steve Dos-Santos Carvalho, Ronan Peyroutou, Sze Chim Lee, Maite M. Moreau, Jean-Michel Blanc, Aysegul Geyser, Chantal Medina, Olivier Thoumine, Sandra Beer-Hammer, Thomas B. Friedman, Lukas Rüttiger, Andrew Forge, Bernd Nürnberg, Nathalie Sans, Mireille Montcouquiol.
  Nat Comms. 2017-04-07. 8 : 14907.
  10.1038/ncomms14907
• Wnt proteins contribute to neuromuscular junction formation through distinct signaling pathways
  Julien Messéant, Jérôme Ezan, Perrine Delers, Konstantin Glebov, Carmen Marchiol, Franck Lager, Gilles Renault, Fadel Tissir, Mireille Montcouquiol, Nathalie Sans, Claire Legay, Laure Strochlic.
  Development. 2017-03-27. 144(9) : 1712-1724.
  10.1242/dev.146167
• Activity-Dependent Neuroplasticity Induced by an Enriched Environment Reverses Cognitive Deficits in Scribble Deficient Mouse.
  Muna L Hilal, Maité M Moreau, Claudia Racca, Vera L Pinheiro, Nicolas H Piguel, Marie-Josée Santoni, Steve Dos Santos Carvalho, Jean-Michel Blanc, Yah-Se K Abada, Ronan Peyroutou, Chantal Medina, Hélène Doat, Thomas Papouin, Laurent Vuillard, Jean-Paul Borg, Rivka Rachel, Aude Panatier, Mireille Montcouquiol, Stéphane H R Oliet, Nathalie Sans.
  Cerebral Cortex. 2016-11-22. 27(12) : 5635-5651.
  10.1093/cercor/bhw333
• Planar Cell Polarity Gene Mutations in Autism Spectrum Disorder, Intellectual Disabilities, and Related Deletion/Duplication Syndromes
  Nathalie Sans, Jérôme Ezan, Maïté M. Moreau, Mireille Montcouquiol.
  Neuronal and Synaptic Dysfunction in Autism Spectrum Disorder and Intellectual Disability. 2016-01-01. : 189-219.
  10.1016/b978-0-12-800109-7.00013-3
• The multiple links between cilia and planar cell polarity | Les liens multiples entre les cils et la polarité planaire cellulaire
  Jérôme Ezan, Mireille Montcouquiol.
  Med Sci (Paris). 2014-11-01. 30(11) : 1004-1010.
  10.1051/medsci/20143011015
• Scribble1/AP2 complex coordinates NMDA receptor endocytic recycling.
  Nicolas H. Piguel, Sabine Fievre, Jean-Michel Blanc, Mario Carta, Maïté M. Moreau, Enora Moutin, Vera L. Pinheiro, Chantal Medina, Jerome Ezan, Léa Lasvaux, François Loll, Christelle M. Durand, Kai Chang, Ronald S. Petralia, Robert J. Wenthold, F. Anne Stephenson, Laurent Vuillard, Hervé Darbon, Julie Perroy, Christophe Mulle, Mireille Montcouquiol, Claudia Racca, Nathalie Sans.
  Cell Reports. 2014-10-01. 9(2) : 712-727.
  10.1016/j.celrep.2014.09.017
• A dual role for planar cell polarity genes in ciliated cells.
  C. Boutin, P. Labedan, J. Dimidschstein, F. Richard, H. Cremer, P. Andre, Y. Yang, M. Montcouquiol, A. M. Goffinet, F. Tissir.
  Proceedings of the National Academy of Sciences. 2014-07-14. 111(30) : E3129-E3138.
  10.1073/pnas.1404988111
• Primary cilium migration depends on G-protein signalling control of subapical cytoskeleton
  Jerome Ezan, Léa Lasvaux, Aysegul Gezer, Ana Novakovic, Helen May-Simera, Edwige Belotti, Anne-Catherine Lhoumeau, Lutz Birnbaumer, Sandra Beer-Hammer, Jean-Paul Borg, André Le Bivic, Bernd Nürnberg, Nathalie Sans, Mireille Montcouquiol.
  Nat Cell Biol. 2013-08-11. 15(9) : 1107-1115.
  10.1038/ncb2819
• The human PDZome: a gateway to PSD95-Disc large-zonula occludens (PDZ)-mediated functions.
  Edwige Belotti, Jolanta Polanowska, Avais M. Daulat, Stéphane Audebert, Virginie Thomé, Jean-Claude Lissitzky, Frédérique Lembo, Karim Blibek, Shizue Omi, Nicolas Lenfant, Akanksha Gangar, Mireille Montcouquiol, Marie-Josée Santoni, Michael Sebbagh, Michel Aurrand-Lions, Stéphane Angers, Laurent Kodjabachian, Jérome Reboul, Jean-Paul Borg.
  Mol Cell Proteomics. 2013-05-30. 12(9) : 2587-2603.
  10.1074/mcp.o112.021022
• Revisiting planar cell polarity in the inner ear
  Jérôme Ezan, Mireille Montcouquiol.
  Seminars in Cell & Developmental Biology. 2013-05-01. 24(5) : 499-506.
  10.1016/j.semcdb.2013.03.012
• Molecular Characterisation of Endogenous Vangl2/Vangl1 Heteromeric Protein Complexes
  Edwige Belotti, Tania M. Puvirajesinghe, Stéphane Audebert, Emilie Baudelet, Luc Camoin, Michel Pierres, Lea Lasvaux, Géraldine Ferracci, Mireille Montcouquiol, Jean-Paul Borg.
  PLoS ONE. 2012-09-28. 7(9) : e46213.
  10.1371/journal.pone.0046213
• Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear
  A. P. Giese, J. Ezan, L. Wang, L. Lasvaux, F. Lembo, C. Mazzocco, E. Richard, J. Reboul, J.-P. Borg, M. W. Kelley, N. Sans, J. Brigande, M. Montcouquiol.
  Development. 2012-09-18. 139(20) : 3775-3785.
  10.1242/dev.074229
• Dishevelled stabilization by the ciliopathy protein rpgrip1l is essential for planar cell polarity
  Alexia Mahuzier, Helori-Mael Gaudé, Valentina Grampa, Isabelle Anselme, Flora Silbermann, Margot Leroux-Berger, Delphine Delacour, Jerome Ezan, Mireille Montcouquiol, Sophie Saunier, Sylvie Schneider-Maunoury, Christine Vesque.
  J Cell Biol. 2012-08-27. 198(5) : 927-940.
  10.1083/jcb.201111009
• Dishevelled stablisation at the cilium by RPGRIP1L is essential for planar cell polarity.
  S Schneider-Maunoury, A Mahuzier, HM Gaudé, I Anselme, F Silbermann, M Leroux-Berger, M Montcouquiol, S Saunier, C Vesque.
  Cilia. 2012-01-01. 1(Suppl 1) : O21.
  10.1186/2046-2530-1-s1-o21
• SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism.
  C M Durand, J Perroy, F Loll, D Perrais, L Fagni, T Bourgeron, M Montcouquiol, N Sans.
  Mol Psychiatry. 2011-05-24. 17(1) : 71-84.
  10.1038/mp.2011.57
• The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function.
  M. M. Moreau, N. Piguel, T. Papouin, M. Koehl, C. M. Durand, M. E. Rubio, F. Loll, E. M. Richard, C. Mazzocco, C. Racca, S. H. R. Oliet, D. Nora Abrous, M. Montcouquiol, N. Sans.
  Journal of Neuroscience. 2010-07-21. 30(29) : 9738-9752.
  10.1523/jneurosci.6007-09.2010
• Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal hydrocephalus
  Fadel Tissir, Yibo Qu, Mireille Montcouquiol, Libing Zhou, Kouji Komatsu, Dongbo Shi, Toshihiko Fujimori, Jason Labeau, Donatienne Tyteca, Pierre Courtoy, Yves Poumay, Tadashi Uemura, Andre M Goffinet.
  Nat Neurosci. 2010-05-16. 13(6) : 700-707.
  10.1038/nn.2555
• Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia
  Boris Guirao, Alice Meunier, Stéphane Mortaud, Andrea Aguilar, Jean-Marc Corsi, Laetitia Strehl, Yuki Hirota, Angélique Desoeuvre, Camille Boutin, Young-Goo Han, Zaman Mirzadeh, Harold Cremer, Mireille Montcouquiol, Kazunobu Sawamoto, Nathalie Spassky.
  Nat Cell Biol. 2010-05-01. 12(5) : 520-520.
  10.1038/ncb0510-520a
• Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia
  Boris Guirao, Alice Meunier, Stéphane Mortaud, Andrea Aguilar, Jean-Marc Corsi, Laetitia Strehl, Yuki Hirota, Angélique Desoeuvre, Camille Boutin, Young-Goo Han, Zaman Mirzadeh, Harold Cremer, Mireille Montcouquiol, Kazunobu Sawamoto, Nathalie Spassky.
  Nat Cell Biol. 2010-03-21. 12(4) : 341-350.
  10.1038/ncb2040
• Planar cell polarity defects and defective Vangl2 trafficking in mutants for the COPII gene Sec24b.
  C. Wansleeben, H. Feitsma, M. Montcouquiol, C. Kroon, E. Cuppen, F. Meijlink.
  Development. 2010-03-09. 137(7) : 1067-1073.
  10.1242/dev.041434
• Maintained Expression of the Planar Cell Polarity Molecule Vangl2 and Reformation of Hair Cell Orientation in the Regenerating Inner Ear
  Mark E. Warchol, Mireille Montcouquiol.
  JARO. 2010-02-23. 11(3) : 395-406.
  10.1007/s10162-010-0209-4
• Scrib regulates PAK activity during the cell migration process.
  Sébastien Nola, Michael Sebbagh, Sylvie Marchetto, Naël Osmani, Claire Nourry, Stéphane Audebert, Christel Navarro, Rivka Rachel, Mireille Montcouquiol, Nathalie Sans, Sandrine Etienne-Manneville, Jean-Paul Borg, Marie-Josée Santoni.
  Human Molecular Genetics. 2008-08-20. 17(22) : 3552-3565.
  10.1093/hmg/ddn248
• Jxc1/Sobp, encoding a nuclear zinc finger protein, is critical for cochlear growth, cell fate, and patterning of the organ of corti.
  Z. Chen, M. Montcouquiol, R. Calderon, N. A. Jenkins, N. G. Copeland, M. W. Kelley, K. Noben-Trauth.
  Journal of Neuroscience. 2008-06-25. 28(26) : 6633-6641.
  10.1523/jneurosci.1280-08.2008
• Detection of Planar Polarity Proteins in Mammalian Cochlea
  Mireille Montcouquiol, Jennifer M. Jones, Nathalie Sans.
  Methods in Molecular Biology. 2008-01-01. : 207-219.
  10.1007/978-1-59745-249-6_16
• Fgf8 induces pillar cell fate and regulates cellular patterning in the mammalian cochlea
  B. E. Jacques, M. E. Montcouquiol, E. M. Layman, M. Lewandoski, M. W. Kelley.
  Development. 2007-07-18. 134(16) : 3021-3029.
  10.1242/dev.02874
• Regulation of cell fate and patterning in the mammalian cochlea
  Matthew W. Kelley, Mireille Montcouquiol, Chad Woods, Jennifer E. Jones, Bonnie E. Jacques, Chandrakala Puligilla, Dabdoub, Elizabeth C. Driver.
  Developmental Biology. 2007-06-01. 306(1) : 315-316.
  10.1016/j.ydbio.2007.03.119
• Proliferative responses to growth factors decline rapidly during postnatal maturation of mammalian hair cell epithelia
  Rende Gu, Mireille Montcouquiol, Mark Marchionni, Jeffrey T. Corwin.
  European Journal of Neuroscience. 2007-04-05. 25(5) : 1363-1372.
  10.1111/j.1460-9568.2007.05414.x
• Lhx3, a LIM domain transcription factor, is regulated by Pou4f3 in the auditory but not in the vestibular system
  Ronna Hertzano, Amiel A. Dror, Mireille Montcouquiol, Zubair M. Ahmed, Buffy Ellsworth, Sally Camper, Thomas B. Friedman, Matthew W. Kelley, Karen B. Avraham.
  European Journal of Neuroscience. 2007-02-26. 25(4) : 999-1005.
  10.1111/j.1460-9568.2007.05332.x
• Polarité planaire chez les Mammifères : similitudes et divergences avec la Drosophile
  Mireille Montcouquiol.
  J. Soc. Biol.. 2007-01-01. 201(1) : 61-68.
  10.1051/jbio:2007007
• Noncanonical Wnt signaling and neural polarity.
  Mireille Montcouquiol, E. Bryan Crenshaw, Matthew W. Kelley.
  Annu. Rev. Neurosci.. 2006-07-21. 29(1) : 363-386.
  10.1146/annurev.neuro.29.051605.112933
• Asymmetric Localization of Vangl2 and Fz3 Indicate Novel Mechanisms for Planar Cell Polarity in Mammals
  Montcouquiol M, Sans N, Huss D, Kach J, Dickman JD, Forge A, Rachel RA, Copeland NG, Jenkins NA, Bogani D, Murdoch J, Warchol ME, Wenthold RJ, Kelley MW..
  Journal of Neuroscience. 2006-05-10. 26(19) : 5265-5275.
  10.1523/jneurosci.4680-05.2006
• Inhibitors of differentiation and DNA binding (Ids) regulate Math1 and hair cell formation during the development of the organ of Corti.
  J. M. Jones.
  Journal of Neuroscience. 2006-01-11. 26(2) : 550-558.
  10.1523/jneurosci.3859-05.2006
• Planar cell polarity in the vertebrate inner ear
  Alain Dabdoub, Mireille Montcouquiol, Matthew W. Kelley.
  Planar Cell Polarization During Development. 2005-01-01. : 107-130.
  10.1016/s1574-3349(05)14006-x
• Math1 regulates development of the sensory epithelium in the mammalian cochlea.
  Chad Woods, Mireille Montcouquiol, Matthew W Kelley.
  Nat Neurosci. 2004-11-07. 7(12) : 1310-1318.
  10.1038/nn1349
• Transcription profiling of inner ears from Pou4f3(ddl/ddl) identifies Gfi1 as a target of the Pou4f3 deafness gene.
  Ronna Hertzano, Mireille Montcouquiol, Sharon Rashi-Elkeles, Rani Elkon, Raif Yücel, Wayne N. Frankel, Gideon Rechavi, Tarik Möröy, Thomas B. Friedman, Matthew W. Kelley, Karen B. Avraham.
  Human Molecular Genetics. 2004-07-14. 13(18) : 2143-2153.
  10.1093/hmg/ddh218
• Wnt signaling mediates reorientation of outer hair cell stereociliary bundles in the mammalian cochlea.
  Alain Dabdoub, Maura J. Donohue, Angela Brennan, Vladimir Wolf, Mireille Montcouquiol, David A. Sassoon, Jen-Chih Hseih, Jeffrey S. Rubin, Patricia C. Salinas, Matthew W. Kelley.
  Development. 2003-06-01. 130(11) : 2375-2384.
  10.1242/dev.00448
• Identification of Vangl2 and Scrb1 as planar polarity genes in mammals.
  Mireille Montcouquiol, Rivka A. Rachel, Pamela J. Lanford, Neal G. Copeland, Nancy A. Jenkins, Matthew W. Kelley.
  Nature. 2003-04-30. 423(6936) : 173-177.
  10.1038/nature01618
• Regeneration in avian hair cell epithelia: identification of intracellular signals required for S-phase entry
  Mark C. Witte, Mireille Montcouquiol, Jeffrey T. Corwin.
  European Journal of Neuroscience. 2001-09-01. 14(5) : 829-838.
  10.1046/j.0953-816x.2001.01695.x
• Postnatal developmental changes in AMPA and NMDA receptors in the rat vestibular nuclei.
  Nathalie A Sans, Mireille E Montcouquiol, Jacqueline Raymond.
  Developmental Brain Research. 2000-09-01. 123(1) : 41-52.
  10.1016/s0165-3806(00)00082-1
• Detection and localization of BDNF in vestibular nuclei during the postnatal development of the rat.
  M E. Montcouquiol, N A. Sans, C Travo, A Sans, J Valat.
  NeuroReport. 2000-05-01. 11(7) : 1401-1405.
  10.1097/00001756-200005150-00010
• Régénération postlésionnelle des cellules sensorielles vestibulaires : bilan et espoir.
  S Bartolami, M Montcouquiol, C Travo, A Sans.
  Med Sci (Paris). 2000-01-01. 16(10) : 1073.
  10.4267/10608/1528
• A role for BDNF in early postnatal rat vestibular epithelia maturation: implication of supporting cells.
  M. Montcouquiol, J. Valat, C. Travo, A. Sans.
  Eur J Neurosci. 1998-02-01. 10(2) : 598-606.
  10.1046/j.1460-9568.1998.00070.x
• Short-term response of postnatal rat vestibular neurons following brain-derived neurotrophic factor or neurotrophin-3 application.
  Mireille Montcouquiol, Jean Valat, Cécile Travo, Alain Sans.
  J. Neurosci. Res.. 1997-11-01. 50(3) : 443-449.
  10.1002/(sici)1097-4547(19971101)50:3<443::aid-jnr10>3.3.co;2-8