Venue: CGFB
Hiromitsu Tanaka
Assistant Professor, Department of Biophysics, University of Kyoto, Japan
https://kdb.iimc.kyoto-u.ac.jp
Invited by David Perrais (IINS)
Title
Visualization of the movement and location of neurotransmitter receptors and synaptic vesicle protein with TIRFM
Abstract
Neurotransmitter receptors and synaptic vesicles move dynamically in response to neuronal activity. We have developed an original experimental system to induce the formation of synaptic like membrane on a glass surface and live-cell image synaptic molecules by TIRFM. I will talk about the exocytosis and endocytosis of AMPA receptors during synaptic plasticity or Synaptophysin after presynaptic activation.
Key publications
Hiromitsu Tanaka, Daiki Sakaguchi, Tomoo Hirano, “Amyloid-β oligomers suppress subunit-specific glutamate receptor increase during LTP”, Alzeheimer’s & Dementia: TRCI, vol5, p797-808 (2019)
Shumpei Fujii, Hiromitsu Tanaka, Tomoo Hirano, “Suppression of AMPA receptor exocytosis contributes to hippocampal LTD”, J. Neuroscience, vol38, p5523-5537, 2018
Junichiro Funahashi, Hiromitsu Tanaka, Tomoo Hirano, “Visualization of synchronous or asynchronous release of single synaptic vesicle in active-zone-like membrane formed on neuroligin-coated glass surface”, Front. Cell. Neurosci., vol12, p1-10 (Article 140), 2018
Shumpei Fujii, Hiromitsu Tanaka, Tomoo Hirano, “Detection and characterization of individual endocytosis of AMPA-type glutamate receptor around postsynaptic membrane”, Genes to Cells, vol22, p583-590, 2017
Hiromitsu Tanaka, Shumpei Fujii, Tomoo Hirano, “Live-cell imaging of receptors around postsynaptic membranes”, Nature Protocols., vol9, p76-89, 2014
Hiromitsu Tanaka, Tomoo Hirano, “Visualization of subunit-specific delivery of glutamate receptors to postsynaptic membrane during hippocampal long-term potentiation”, Cell Reports, vol1, p291-298, 2012