Structural Biology and Engineering of Neuronal Proteins
The interests of our team lay at the interface of structural biology, protein engineering, and cellular neuroscience. We aim to understand molecular principles of neuronal signalling in health and disease, and to translate these to the cellular and organismal level.
We use mammalian protein expression, protein chemistry, biophysical methods, X-ray crystallography and cryo-electron microscopy to study the interaction determinants and structures of synaptic protein complexes involved in neurodevelopmental disorders and neuronal disease. We use combinatorial methods and protein engineering to discover novel binders and manipulate protein sequence, structure and function, both to facilitate structural studies as well as enable therapeutic targeting of these complexes.
Mots clés
Adhésion cellulaire et cytosquelette, Alzheimer, Biochemistry, Démences, Nouvelles approches en biologie structurale, Signalisation, Synapse molecular complexesSelected publications
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High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus.
Ester Behiels, Jonathan Elegheert.
Methods in Molecular Biology. 2021-01-01. : 83-104.
DOI: 10.1007/978-1-0716-1406-8_4 -
A synthetic synaptic organizer protein restores glutamatergic neuronal circuits.
Suzuki K, Elegheert J, Song I, Sasakura H, Senkov O, Matsuda K, Kakegawa W, Clayton AJ, Chang VT, Ferrer-Ferrer M, Miura E, Kaushik R, Ikeno M, Morioka Y, Takeuchi Y, Shimada T, Otsuka S, Stoyanov S, Watanabe M, Takeuchi K, Dityatev A, Aricescu AR, Yuzaki M.
Science. . 369 (6507) : 1052-1053.
DOI: 10.1126/science.abb4853 -
Pumping the brakes: suppression of synapse development by MDGA-neuroligin interactions.
Steven A Connor, Jonathan Elegheert, Yicheng Xie, Ann Marie Craig.
Current Opinion in Neurobiology. 2019-08-01. 57 : 71-80.
DOI: 10.1016/j.conb.2019.01.002 -
Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins
Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu.
Nat Protoc. 2018-11-19. 13 (12) : 2991-3017.
DOI: 10.1038/s41596-018-0075-9 -
Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins.
Jonathan Elegheert, Vedrana Cvetkovska, Amber J. Clayton, Christina Heroven, Kristel M. Vennekens, Samuel N. Smukowski, Michael C. Regan, Wanyi Jia, Alexandra C. Smith, Hiro Furukawa, Jeffrey N. Savas, Joris de Wit, Jo Begbie, Ann Marie Craig, A. Radu Aricescu.
Neuron. 2017-09-01. 96 (1) : 242-244.
DOI: 10.1016/j.neuron.2017.07.040 -
Structural basis for integration of GluD receptors within synaptic organizer complexes.
Jonathan Elegheert, Wataru Kakegawa, Jordan E. Clay, Natalie F. Shanks, Ester Behiels, Keiko Matsuda, Kazuhisa Kohda, Eriko Miura, Maxim Rossmann, Nikolaos Mitakidis, Junko Motohashi, Veronica T. Chang, Christian Siebold, Ingo H. Greger, Terunaga Nakagawa, Michisuke Yuzaki, A. Radu Aricescu.
Science. 2016-07-14. 353 (6296) : 295-299.
DOI: 10.1126/science.aae0104 -
Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein.
Eleanor G Healey, Benjamin Bishop, Jonathan Elegheert, Christian H Bell, Sergi Padilla-Parra, Christian Siebold.
Nat Struct Mol Biol. 2015-05-04. 22 (6) : 458-465.
DOI: 10.1038/nsmb.3016 -
Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis
Charlotte H. Coles, Nikolaos Mitakidis, Peng Zhang, Jonathan Elegheert, Weixian Lu, Andrew W. Stoker, Terunaga Nakagawa, Ann Marie Craig, E. Yvonne Jones, A. Radu Aricescu.
Nat Commun. 2014-11-11. 5 (1)
DOI: 10.1038/ncomms6209
Team member(s)
Chercheurs, Praticiens hospitaliers...
Ingénieur(e)s, technicien(ne)s
Post-doctorant(s)
Doctorant(s)
Neuropsychologist(s) and speech therapist(s)
Ingénieur(s) hospitalier(s) et ARC