Lieu : Centre Broca
Defense in french
Thesis supervisor: Laurent Juvin (INCIA)
Title
Consequences of perinatal exposure to titanium dioxide nanoparticles on the development and behavior of mice offspring
Abstract
The use of titanium dioxide nanoparticles (TiO2 NPs) has increased dramatically over the past decades, and these particles are now present in many everyday consumer products. Due to their small size, TiO2 nanoparticles can cross biological barriers and interact negatively with biological tissues. In particular, it has been shown that perinatal exposure of pregnant females to TiO2 nanoparticles can alter the body growth of the offspring. However, the majority of these studies have focused on a relatively restricted duration of exposure, often limited to the prenatal period. Here, we daily exposed female mice from the first day of gestation to the last day of lactation via voluntary food intake of TiO2 nanoparticles. We show disturbances in metabolism and behavior throughout the life of the offspring. In particular, we were able to observe alterations in the behavior of animals during the neonatal period, during the period corresponding to adolescence and finally to adulthood. First of all, newborn animals from exposed litters show a lower growth curve than non-exposed animals. In addition, they exhibit a decreased respiratory rate and an increased likelihood of producing respiratory apneas. In addition, a decrease and a delay in the development of ultrasonic vocalizations are observed in newborn animals. During adolescence, exposed animals show an increase in the production of anxiety-like behaviors, as revealed by open field and dark and light box tests. The use of the three-chamber test also made it possible to reveal a decrease in the interest of the exposed animals towards their congeners. The majority of these behavioral changes are retained into adulthood in both males and females.
Publications
2021 Boulain M, Khsime I, Sourioux M, Thoby-Brisson M, Barrière G, Simmers J, Morin D, Juvin L, Synergistic interaction between sensory inputs and propriospinal signaling underlying quadrupedal locomotion. J Physiol.
2021 Boulain M, Yuan W, Oueghlani Z, Khsime I, Salvi V, Courtand G, Halgand C, Morin D, de Deurwaerdere P, Barrière G & Juvin L, L-DOPA and 5-HTP modulation of air-stepping in newborn rats. J Physiol.
2020 Chagraoui A, Boulain M, Juvin L, Anouar Y, Barrière G & Deurwaerdère P., L-DOPA in Parkinson’s Disease: Looking at the “False” Neurotransmitters and Their Meaning. Int J Mol Sci.
Jury
Mme Anna BENCSIK
M. David BELIN
Mme Muriel DARNAUDERY
M. Olivier PIERREFICHE
M. Laurent Juvin
M. Didier Morin