Venue: Centre Broca
Maxime Cazorla
Institut de Neuroscience de la Timone / INT Neurotechnology Center
Aix Marseille Université
team / ntc / twitter
Invited by Clémentine Bosch Bouju (NutriNeuro)
Title
Studying axon plasticity : from intracellular dynamics to large-scale rewiring
Abstract
Long regarded as a rigid and aging organ, the adult brain is capable of profound reorganization at the macroscopic level of its cerebral circuits. This emerging concept, known as ‘brain rewiring’, postulates that neural circuits keep restructuring in response to neuronal activity. But what makes axonal rewiring efficient in the adult brain? How is it regulated with experience and learning? Alterations in brain connectivity exist in neurodegenerative diseases, such as Huntington’s disease and Amyotrophic Lateral Sclerosis. Could we reverse engineer the rewiring capacity of the brain to restore dysfunctional networks on-demand? In the lab, we address these burning questions by investigating the molecular and cellular underpinnings of axonal remodeling, using an organ-on-a-chip approach, in health and disease.
In this talk, I will present how brain-on-chip microdevices helped us to discover unexpected intracellular mechanisms regulating axonal remodeling. I will also introduce our latest human-based models and technological development to improve biomedical research. Finally, I will briefly talk about RAIL, a photoinducible technology targeting axon remodeling programs to connect and disconnect neural networks on-demand.
Key publications
Cazorla M, de Carvalho FD, Chohan MO, Shegda M, Chuhma N, Rayport S, Ahmari SE, Moore H and Kellendonk C. (2014) Dopamine D2 receptors regulate the anatomical and functional balance of basal ganglia circuitry. Neuron. 2014 Jan 8;81(1):153-64.
Virlogeux A, Moutaux E, Christaller W, Genoux A, Bruyère J, Fino E, Charlot B, Cazorla M and Saudou F. (2018) Reconstituting Corticostriatal Network on‐a‐Chip Reveals the Contribution of the Presynaptic Compartment to Huntington’s Disease. Cell Reports. 2018 Jan 2;22(1):110‐122
Moutaux E, Christaller W, Scaramuzzino C, Genoux A, Charlot B, Cazorla M* and Saudou F*. (2018) Neuronal network maturation differently affects secretory vesicles and mitochondria transport in axons. Scientific Reports. 2018 Sep 7;8(1):13429.
Moutaux E, Charlot B, Genoux A, Saudou F and Cazorla M. (2018) An integrated microfluidic/microelectrode array for the study of activity‐dependent intracellular dynamics in neuronal networks. Lab Chip. 2018 Nov 6;18(22):3425‐3435
Poinsot M, Maurin J, Cesar W and Cazorla M. (2023) Liquid distribution system for a fluidic chip. Eur. Patent.EP/2023/03848