Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons
Mol Neurobiol. 2015-09-16; 53(7): 5000-5012
DOI: 10.1007/s12035-015-9432-7
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1. Mol Neurobiol. 2016 Sep;53(7):5000-12. doi: 10.1007/s12035-015-9432-7. Epub 2015
Sep 16.
Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory
Pyramidal Glutamatergic Neurons.
Louessard M(1), Lacroix A(2)(3)(4), Martineau M(5), Mondielli G(6), Montagne
A(1), Lesept F(1), Lambolez B(2)(3)(4), Cauli B(2)(3)(4), Mothet JP(6), Vivien
D(7), Maubert E(1).
Author information:
(1)INSERM, UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular
Unit, Université Caen Basse-Normandie, GIP Cyceron, Bvd Henri Becquerel, GIP
Cyceron, 14074, Caen, France.
(2)UPMC Univ Paris 06, Institut de Biologie Paris-Seine (IBPS), UM 119,
Neuroscience Paris Seine, Sorbonne Universités, 75005, Paris, France.
(3)CNRS, UMR 8246, Neuroscience Paris Seine, 75005, Paris, France.
(4)INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France.
(5)Department of Cellular Biophysics, Institute for Medical Physics and
Biophysics, University of Muenster, Muenster, Germany.
(6)Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille UMR7286
CNRS, Aix Marseille University, Marseille, France.
(7)INSERM, UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular
Unit, Université Caen Basse-Normandie, GIP Cyceron, Bvd Henri Becquerel, GIP
Cyceron, 14074, Caen, France. .
Although the extracellular serine protease tissue plasminogen activator (tPA) is
involved in pathophysiological processes such as learning and memory, anxiety,
epilepsy, stroke, and Alzheimer’s disease, information about its regional,
cellular, and subcellular distribution in vivo is lacking. In the present study,
we observed, in healthy mice and rats, the presence of tPA in endothelial cells,
oligodendrocytes, mastocytes, and ependymocytes, but not in pericytes, microglial
cells, and astrocytes. Moreover, blockage of the axo-dendritic transport unmasked
tPA expression in neurons of cortical and hippocampal areas. Interestingly,
combined electrophysiological recordings, single-cell reverse transcription
polymerase chain reaction (RT-PCR), and immunohistological analyses revealed that
the presence of tPA is restricted to subsets of excitatory pyramidal
glutamatergic neurons. We further evidenced that tPA is stored in
synaptobrevin-2-positive glutamatergic synaptic vesicles. Based on all these
data, we propose the existence of tPA-ergic neurons in the mature brain.
DOI: 10.1007/s12035-015-9432-7
PMID: 26377106 [Indexed for MEDLINE]