Dietary n-3 and n-6 PUFA enhance DHA incorporation in retinal phospholipids without affecting PGE1 and PGE2 levels
Lipids. 2009-02-26; 44(5): 465-470
DOI: 10.1007/s11745-009-3289-3
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1. Lipids. 2009 May;44(5):465-70. doi: 10.1007/s11745-009-3289-3. Epub 2009 Feb 26.
Dietary n-3 and n-6 PUFA enhance DHA incorporation in retinal phospholipids
without affecting PGE(1) and PGE (2) levels.
Schnebelen C(1), Grégoire S, Pasquis B, Joffre C, Creuzot-Garcher CP, Bron AM,
Bretillon L, Acar N.
Author information:
(1)Eye and Nutrition Research Group, INRA, UMR1129 FLAVIC, 21000, Dijon, France.
The purpose of this study was to determine whether dietary n-3 and n-6 PUFA may
affect retinal PUFA composition and PGE(1) and PGE(2) production. Male Wistar
rats were fed for 3 months with diets containing: (1) 10% eicosapentaenoic acid
(EPA) and 7% docosahexaenoic acid (DHA), or (2) 10% gamma-linolenic acid (GLA),
or (3) 10% EPA, 7% DHA and 10% GLA, or (4) a balanced diet deprived of EPA, DHA,
and GLA. The fatty acid composition of retinal phospholipids was determined by
gas chromatography. Prostaglandin production was measured by enzyme immunoassay.
When compared to rats fed the control diet, the retinal levels of DHA were
increased in rats fed both diets enriched with n-3 PUFA (EPA + DHA and EPA + DHA
+ GLA diets) and decreased in those supplemented with n-6 PUFA only (GLA diet).
The diet enriched with both n-6 and n-3 PUFA resulted in the greatest increase in
retinal DHA. The levels of PGE(1) and PGE(2) were significantly increased in
retinal homogenates of rats fed with the GLA-rich diet when compared with those
of animals fed the control diet. These higher PGE(1) and PGE(2) levels were not
observed in animals fed with EPA + DHA + GLA. In summary, GLA added to EPA + DHA
resulted in the highest retinal DHA content but without increasing retinal PGE(2)
as seen in animals supplemented with GLA only.
DOI: 10.1007/s11745-009-3289-3
PMID: 19242743 [Indexed for MEDLINE]