Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics
Methods in Molecular Biology. 2019-01-01; : 145-155
DOI: 10.1007/978-1-4939-9095-5_11
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Bancelin S(1)(2), Lynch B(3)(4), Bonod-Bidaud C(5)(6), Dokládal P(7), Ruggiero F(5), Allain JM(3)(8), Schanne-Klein MC(9).
Author information:
(1)Laboratoire d’Optique et Biosciences (LOB), Ecole Polytechnique, CNRS, INSERM, Université Paris-Saclay, Palaiseau, France.
(2)Institut Interdisciplinaire de Neurosciences, CNRS, Université de Bordeaux, Bordeaux, France.
(3)Laboratoire de Mécanique des Solides (LMS), Ecole Polytechnique, CNRS, Université Paris-Saclay, Palaiseau, France.
(4)L’Oréal Research and Innovation, Aulnay-sous-Bois, France.
(5)Institut de Génomique Fonctionnelle de Lyon, ENS-Lyon, CNRS UMR 5242, Université de Lyon, Lyon, France.
(6)Tissue Biology and Therapeutic Engineering Unit, LBTI, CNRS UMR 5305, Univ. Lyon, Université Claude Bernard Lyon 1, Lyon, France.
(7)Centre for Mathematical Morphology, MINES ParisTech, PSL Research University, Fontainebleau, France.
(8)Inria, Université Paris-Saclay, Palaiseau, France.
(9)Laboratoire d’Optique et Biosciences (LOB), Ecole Polytechnique, CNRS, INSERM, Université Paris-Saclay, Palaiseau, France.
An important issue in tissue biomechanics is to decipher the relationship between
the mechanical behavior at macroscopic scale and the organization of the collagen
fiber network at microscopic scale. Here, we present a protocol to combine
traction assays with multiphoton microscopy in ex vivo murine skin. This
multiscale approach provides simultaneously the stress/stretch response of a skin
biopsy and the collagen reorganization in the dermis by use of second harmonic
generation (SHG) signals and appropriate image processing.