Absence of accessory subunit in the DNA polymerase γ purified from yeast mitochondria
1. Mitochondrion. 2004 Jun;4(1):13-20.
Absence of accessory subunit in the DNA polymerase gamma purified from yeast
mitochondria.
Lucas P(1), Lasserre JP, Plissonneau J, Castroviejo M.
Author information:
(1)Laboratoire Reger, UMR 5097, Université Victor Segalen Bordeaux 2, Bâtiment
3A, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
In yeast and animals, replication of the mitochondrial genome is carried out by
the DNA polymerase gamma. In mammals this polymerase is composed of a catalytic
and an accessory subunit. Yeast DNA polymerase gamma was purified over 6600-fold
from mitochondria. The catalytic polypeptide of this enzyme was identified as a
135-kDa protein by a photochemical crosslinking procedure and its native
molecular weight was estimated between 120 and 140 kDa by gel filtration and
glycerol gradient sedimentation. These results indicate that yeast DNA polymerase
gamma contains only one subunit and thus has a different quaternary structure
from its counterpart in animals.
DOI: 10.1016/j.mito.2004.04.001
PMID: 16120369