Tryptophan Metabolic Pathways Are Altered in Obesity and Are Associated With Systemic Inflammation
Front. Immunol.. 2020-04-15; 11:
DOI: 10.3389/fimmu.2020.00557
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1. Front Immunol. 2020 Apr 15;11:557. doi: 10.3389/fimmu.2020.00557. eCollection
2020.
Tryptophan Metabolic Pathways Are Altered in Obesity and Are Associated With
Systemic Inflammation.
Cussotto S(1), Delgado I(1), Anesi A(2), Dexpert S(1), Aubert A(1), Beau C(3),
Forestier D(3), Ledaguenel P(3), Magne E(3), Mattivi F(2)(4), Capuron L(1).
Author information:
(1)University of Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, Bordeaux,
France.
(2)Department of Food Quality and Nutrition, Research and Innovation Centre,
Fondazione Edmund Mach (FEM), San Michele all’Adige, Italy.
(3)Service de Chirurgie Digestive et Parietale, Clinique Tivoli, Bordeaux, and
Clinique Jean Villar, Bruges, France.
(4)Department of Cellular, Computational and Integrative Biology (CIBIO),
University of Trento, Trento, Italy.
Background: Obesity is a condition with a complex pathophysiology characterized
by both chronic low-grade inflammation and changes in the gut microbial
ecosystem. These alterations can affect the metabolism of tryptophan (TRP), an
essential amino acid and precursor of serotonin (5-HT), kynurenine (KYN), and
indoles. This study aimed to investigate alterations in KYN and
microbiota-mediated indole routes of TRP metabolism in obese subjects relatively
to non-obese controls and to determine their relationship with systemic
inflammation. Methods: Eighty-five obese adults (avg. BMI = 40.48) and 42
non-obese control individuals (avg. BMI = 24.03) were recruited. Plasma levels
of TRP catabolites were assessed using Ultra High Performance Liquid
Chromatography-ElectroSpray-Ionization-Tandem Mass Spectrometry. High-sensitive
C-reactive protein (hsCRP) and high-sensitive interleukin 6 (hsIL-6) were
measured in the serum as markers of systemic inflammation using enzyme-linked
immunosorbent assay. Results: Both KYN and microbiota-mediated indole routes of
TRP metabolism were altered in obese subjects, as reflected in higher KYN/TRP
ratio and lower 5-HT and indoles levels, relatively to non-obese controls.
HsIL-6 and hsCRP were increased in obesity and were overall associated with TRP
metabolic pathways alterations. Conclusion: These results indicate for the first
time that KYN and indole TRP metabolic pathways are concomitantly altered in
obese subjects and highlight their respective associations with obesity-related
systemic inflammation.
Copyright © 2020 Cussotto, Delgado, Anesi, Dexpert, Aubert, Beau, Forestier,
Ledaguenel, Magne, Mattivi and Capuron.
DOI: 10.3389/fimmu.2020.00557
PMCID: PMC7174689
PMID: 32351500 [Indexed for MEDLINE]