Imaging mitochondrial calcium dynamics in the central nervous system

Serrat R(1), Oliveira-Pinto A(1), Marsicano G(1), Pouvreau S(2).

Author information:
(1)Institut National de la Santé et de la Recherche Médicale (INSERM), U1215
NeuroCentre Magendie, France; University of Bordeaux, Bordeaux 33077, France.
(2)Institut National de la Santé et de la Recherche Médicale (INSERM), U1215
NeuroCentre Magendie, France; University of Bordeaux, Bordeaux 33077, France.
Electronic address: .

Mitochondrial calcium handling is a particularly active research area in the
neuroscience field, as it plays key roles in the regulation of several functions
of the central nervous system, such as synaptic transmission and plasticity,
astrocyte calcium signaling, neuronal activity… In the last few decades, a panel
of techniques have been developed to measure mitochondrial calcium dynamics,
relying mostly on photonic microscopy, and including synthetic sensors, hybrid
sensors and genetically encoded calcium sensors. The goal of this review is to
endow the reader with a deep knowledge of the historical and latest tools to
monitor mitochondrial calcium events in the brain, as well as a comprehensive
overview of the current state of the art in brain mitochondrial calcium
signaling. We will discuss the main calcium probes used in the field, their
mitochondrial targeting strategies, their key properties and major drawbacks. In
addition, we will detail the main roles of mitochondrial calcium handling in
neuronal tissues through an extended report of the recent studies using
mitochondrial targeted calcium sensors in neuronal and astroglial cells, in
vitro and in vivo.

Copyright © 2022 Elsevier B.V. All rights reserved.

DOI: 10.1016/j.jneumeth.2022.109560
PMID: 35320763 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus