Imaging of post-synaptic membrane trafficking in neuronal dendrites: progress, limitations, and new developments

Neurophotonics. 2023 Oct;10(4):044404. doi: 10.1117/1.NPh.10.4.044404. Epub 2023 Aug 4.

Abstract

Membrane trafficking of post-synaptic cargo is a key determinant of synaptic transmission and synaptic plasticity. We describe here the latest developments in visualizing individual exocytosis and endocytosis events in neurons using pH-sensitive tags. We show how these tools help decipher the spatial and temporal regulation of membrane trafficking steps during synaptic plasticity.

Keywords: endocytosis; exocytosis; live cell fluorescence imaging; membrane trafficking; synaptic plasticity.